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Transcript levels (designated as median fluorescence intensity (MFI) of endogenous rat Snca and mutated humanized SNCA genes were quantified in the right brain hemisphere samples using the QuantiGene Plex assay and normalized to the geometric mean of mRNA expression levels of housekeeping genes Hmbs , Ppib , and G apdh in the cortex (A, B), hippocampus (C, D), striatum (E, F), substantia nigra (G, H), and cerebellum (I, J). Data are presented as box-whisker plots. Data were analyzed using ordinary two-way ANOVA with genotype and age as independent factors. If the genotype × age effect was significant, pairwise comparisons within the same age group were done by the Holm–Šídák’s multiple comparisons test as follows: *** P < 0.001 **** P < 0.0001. If the factor interaction effect was not significant, main genotype effect was illustrated as follows: #### P < 0.0001.

Journal: PLOS One

Article Title: Expression of human A53T alpha-synuclein without endogenous rat alpha-synuclein fails to elicit Parkinson’s disease-related phenotypes in a novel humanized rat model

doi: 10.1371/journal.pone.0329823

Figure Lengend Snippet: Transcript levels (designated as median fluorescence intensity (MFI) of endogenous rat Snca and mutated humanized SNCA genes were quantified in the right brain hemisphere samples using the QuantiGene Plex assay and normalized to the geometric mean of mRNA expression levels of housekeeping genes Hmbs , Ppib , and G apdh in the cortex (A, B), hippocampus (C, D), striatum (E, F), substantia nigra (G, H), and cerebellum (I, J). Data are presented as box-whisker plots. Data were analyzed using ordinary two-way ANOVA with genotype and age as independent factors. If the genotype × age effect was significant, pairwise comparisons within the same age group were done by the Holm–Šídák’s multiple comparisons test as follows: *** P < 0.001 **** P < 0.0001. If the factor interaction effect was not significant, main genotype effect was illustrated as follows: #### P < 0.0001.

Article Snippet: Thereafter, RNAlater was removed and samples were stored at −80 °C until used for RNA transcript analyses using QuantiGene branched DNA (bDNA) technology (ThermoFisher Scientific, Waltham, MA, USA).

Techniques: Fluorescence, Plex Assay, Expressing, Whisker Assay

The QuantiGene Plex assay that utilized upstream and downstream probe sets for rat Snca showed specific signals (designated as Normalized MFI) only in samples from WT rats but not in samples from aSyn KO animals in the cortex (A), hippocampus (B), substantia nigra (C) and cerebellum (D). Data were analyzed using ordinary two-way ANOVA with genotype and age as independent factors. If the genotype × age effect was significant, pairwise comparisons within the same age group were done by the Holm–Šídák’s multiple comparisons test as follows: **** P < 0.0001. If the factor interaction effect was not significant, main genotype effect was illustrated as follows: #### P < 0.0001.

Journal: PLOS One

Article Title: Expression of human A53T alpha-synuclein without endogenous rat alpha-synuclein fails to elicit Parkinson’s disease-related phenotypes in a novel humanized rat model

doi: 10.1371/journal.pone.0329823

Figure Lengend Snippet: The QuantiGene Plex assay that utilized upstream and downstream probe sets for rat Snca showed specific signals (designated as Normalized MFI) only in samples from WT rats but not in samples from aSyn KO animals in the cortex (A), hippocampus (B), substantia nigra (C) and cerebellum (D). Data were analyzed using ordinary two-way ANOVA with genotype and age as independent factors. If the genotype × age effect was significant, pairwise comparisons within the same age group were done by the Holm–Šídák’s multiple comparisons test as follows: **** P < 0.0001. If the factor interaction effect was not significant, main genotype effect was illustrated as follows: #### P < 0.0001.

Article Snippet: Thereafter, RNAlater was removed and samples were stored at −80 °C until used for RNA transcript analyses using QuantiGene branched DNA (bDNA) technology (ThermoFisher Scientific, Waltham, MA, USA).

Techniques: Plex Assay